| pubmed-article:3551834 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3551834 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:3551834 | lifeskim:mentions | umls-concept:C0018150 | lld:lifeskim |
| pubmed-article:3551834 | lifeskim:mentions | umls-concept:C0020202 | lld:lifeskim |
| pubmed-article:3551834 | lifeskim:mentions | umls-concept:C0597979 | lld:lifeskim |
| pubmed-article:3551834 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:3551834 | lifeskim:mentions | umls-concept:C0022169 | lld:lifeskim |
| pubmed-article:3551834 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3551834 | pubmed:dateCreated | 1987-5-20 | lld:pubmed |
| pubmed-article:3551834 | pubmed:abstractText | Isoelectric focusing and molecular hybridization with a TEM DNA probe were used to screen for TEM beta-lactamase in 328 bacterial isolates representing 11 gram-negative genera. The TEM enzyme was detected in 50% of isolates, and nine additional types of beta-lactamase could be identified in 36.9% of isolates. The TEM gene was detected in 53.6% of isolates. The results obtained by both methods were concordant in 92.7% of the entire sample. In situ colony hybridization with a specific probe therefore appears to be a convenient method to screen rapidly for the presence of homologous genetic sequences among a large number of isolates. Positive hybridization was observed for 16 isolates in which no TEM beta-lactamase was detected by isoelectric focusing. The significance of this hybridization remains to be determined. | lld:pubmed |
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| pubmed-article:3551834 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3551834 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3551834 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3551834 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3551834 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:3551834 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:CookseyR CRC | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:Michel-Briand... | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:JouvenotMM | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:MouginCC | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:AdessiG LGL | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:DeschaseauxM... | lld:pubmed |
| pubmed-article:3551834 | pubmed:author | pubmed-author:RoyezMM | lld:pubmed |
| pubmed-article:3551834 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3551834 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:3551834 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3551834 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3551834 | pubmed:pagination | 300-5 | lld:pubmed |
| pubmed-article:3551834 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3551834 | pubmed:meshHeading | pubmed-meshheading:3551834-... | lld:pubmed |
| pubmed-article:3551834 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3551834 | pubmed:articleTitle | Molecular hybridization versus isoelectric focusing to determine TEM-type beta-lactamases in gram-negative bacteria. | lld:pubmed |
| pubmed-article:3551834 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3551834 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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