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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-5-20
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pubmed:abstractText |
Isoelectric focusing and molecular hybridization with a TEM DNA probe were used to screen for TEM beta-lactamase in 328 bacterial isolates representing 11 gram-negative genera. The TEM enzyme was detected in 50% of isolates, and nine additional types of beta-lactamase could be identified in 36.9% of isolates. The TEM gene was detected in 53.6% of isolates. The results obtained by both methods were concordant in 92.7% of the entire sample. In situ colony hybridization with a specific probe therefore appears to be a convenient method to screen rapidly for the presence of homologous genetic sequences among a large number of isolates. Positive hybridization was observed for 16 isolates in which no TEM beta-lactamase was detected by isoelectric focusing. The significance of this hybridization remains to be determined.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-1167043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-13945492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-15828191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-2989147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-377324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-378931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-379573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-383387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-385575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-3876073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-3925874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4208895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4553144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4599117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4614864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4628744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4888148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4919751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-4945193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6100076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6109327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6231593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6381467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6392327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6423610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6607250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-6996612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-7009583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-7045250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-806258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-807430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3551834-807678
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
300-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3551834-DNA, Bacterial,
pubmed-meshheading:3551834-Escherichia coli,
pubmed-meshheading:3551834-Genes, Bacterial,
pubmed-meshheading:3551834-Gram-Negative Bacteria,
pubmed-meshheading:3551834-Humans,
pubmed-meshheading:3551834-Isoelectric Focusing,
pubmed-meshheading:3551834-Nucleic Acid Hybridization,
pubmed-meshheading:3551834-Plasmids,
pubmed-meshheading:3551834-beta-Lactamases
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pubmed:year |
1987
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pubmed:articleTitle |
Molecular hybridization versus isoelectric focusing to determine TEM-type beta-lactamases in gram-negative bacteria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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