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rdf:type |
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lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0004083,
umls-concept:C0023767,
umls-concept:C0023810,
umls-concept:C0037791,
umls-concept:C0204727,
umls-concept:C0205369,
umls-concept:C0205409,
umls-concept:C0521009,
umls-concept:C0591833,
umls-concept:C1880022
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pubmed:issue |
4
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pubmed:dateCreated |
1987-4-30
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pubmed:abstractText |
Somatic cell hybrids secreting monoclonal antibodies against the core-glycolipid portion of enterobacterial endotoxin were derived from mice immunized with Escherichia coli J5 or Salmonella minnesota R595 heat-killed organisms or lipopolysaccharide (LPS). Eight antibodies were selected for their ability to cross-react with several members of a panel of gram-negative bacterial antigens in a radioimmunoassay. This panel represented five genera and two families of organisms: E. coli O111:B4, E. coli O55:B5, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella minnesota, and Serratia marcescens. The binding sites for six of the antibodies were unequivocally localized within the lipid A moiety of the endotoxin molecule by using the radioimmunoassay on LPS and free lipid A. The anti-lipid A antibodies were further characterized for their ability to interact with LPS variants by using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunostaining procedure. The monoclonal antibodies bound almost exclusively to the low-molecular-weight species of LPS on the polyacrylamide gel. These components corresponded to LPS isolated from rough strains of organisms (strains which lack O-specific carbohydrate). These results suggested that the cross-reactive component of antisera raised against rough mutants of gram-negative bacteria contain antibodies of lipid A specificity. Moreover, the determinant within the lipid A moiety of LPS may have been accessible to the monoclonal antibodies only in those endotoxin molecules on the outer membrane surface which lack the O-specific carbohydrate.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-113458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-1172191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-13578996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-2431039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-2582064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-30693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-330775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-330776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-3514463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-356058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-3856860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-3886631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-3889175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-3895540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-396770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-4164249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-4558866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-4577881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-4611919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-4622185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-4944989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6176137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6185599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6206544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6209222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6358257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6381310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6469268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6752708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-6799587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-69613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-7031250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-7048594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-7288213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-778329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3549565-825377
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
899-908
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3549565-Animals,
pubmed-meshheading:3549565-Antibodies, Bacterial,
pubmed-meshheading:3549565-Antibodies, Monoclonal,
pubmed-meshheading:3549565-Antibody Specificity,
pubmed-meshheading:3549565-Antigens, Bacterial,
pubmed-meshheading:3549565-Escherichia coli,
pubmed-meshheading:3549565-Female,
pubmed-meshheading:3549565-Gram-Negative Bacteria,
pubmed-meshheading:3549565-Hybridomas,
pubmed-meshheading:3549565-Immunosorbent Techniques,
pubmed-meshheading:3549565-Klebsiella pneumoniae,
pubmed-meshheading:3549565-Lipid A,
pubmed-meshheading:3549565-Mice,
pubmed-meshheading:3549565-Mice, Inbred Strains,
pubmed-meshheading:3549565-Radioimmunoassay,
pubmed-meshheading:3549565-Salmonella,
pubmed-meshheading:3549565-Salmonella typhimurium,
pubmed-meshheading:3549565-Species Specificity
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation and characterization of murine monoclonal antibodies specific for gram-negative bacterial lipopolysaccharide: association of cross-genus reactivity with lipid A specificity.
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pubmed:publicationType |
Journal Article
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