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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1987-5-13
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pubmed:abstractText |
Addition of insulin to isolated rat hepatocytes prelabeled with [32P]phosphate inhibited glucagon-dependent phospholipid methyltransferase phosphorylation and activation. Insulin alone had no effect on either the phosphorylation of the enzyme or on its activity. The effect of insulin on glucagon-dependent phospholipid methyltransferase phosphorylation was dose-dependent and occurred at physiological doses of the hormone (10(-11)-10(-10) M). Analysis of 32P-labeled peptides after digestion with trypsin revealed only one site of phosphorylation regulated by glucagon (10(-8) M) in isolated rat hepatocytes. This site, as analyzed by HPLC and thin-layer chromatography, coincided with that phosphorylated by the cAMP-dependent protein kinase using purified rat liver phospholipid methyltransferase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
928
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
92-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3548831-Animals,
pubmed-meshheading:3548831-Enzyme Activation,
pubmed-meshheading:3548831-Glucagon,
pubmed-meshheading:3548831-Insulin,
pubmed-meshheading:3548831-Liver,
pubmed-meshheading:3548831-Methyltransferases,
pubmed-meshheading:3548831-Phosphatidyl-N-Methylethanolamine N-Methyltransferase,
pubmed-meshheading:3548831-Phosphatidylethanolamine N-Methyltransferase,
pubmed-meshheading:3548831-Phosphoproteins,
pubmed-meshheading:3548831-Phosphorylation,
pubmed-meshheading:3548831-Rats
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pubmed:year |
1987
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pubmed:articleTitle |
Inhibition by insulin of glucagon-dependent phospholipid methyltransferase phosphorylation in rat hepatocytes.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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