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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1987-4-6
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pubmed:databankReference |
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pubmed:abstractText |
A 1.5-kilobase-pair fragment of DNA that contains the lysostaphin gene from Staphylococcus simulans and its flanking sequences has been cloned and completely sequenced. The gene encodes a preproenzyme of Mr 42,000. The NH2-terminal sequence of the preproenzyme is composed of a signal peptide followed by seven tandem repeats of a 13-amino acid sequence. Conversion of prolysostaphin to the mature enzyme occurs extracellularly in cultures of S. simulans and involves removal of the NH2-terminal portion of the proenzyme that contains the tandem repeats. The high degree of homology of the repeats suggests that they have arisen by duplication of a 39-base-pair sequence of DNA. In S. simulans, the lysostaphin gene is present on a large beta-lactamase plasmid.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3547405-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3547405-1195397,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3547405-927197
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1127-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3547405-Amino Acid Sequence,
pubmed-meshheading:3547405-Base Sequence,
pubmed-meshheading:3547405-Cloning, Molecular,
pubmed-meshheading:3547405-Escherichia coli,
pubmed-meshheading:3547405-Genes,
pubmed-meshheading:3547405-Genes, Bacterial,
pubmed-meshheading:3547405-Lysostaphin,
pubmed-meshheading:3547405-Staphylococcus,
pubmed-meshheading:3547405-Transcription, Genetic
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pubmed:year |
1987
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pubmed:articleTitle |
Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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