| pubmed-article:3545823 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C0392918 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C0035681 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C1710236 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C1998548 | lld:lifeskim |
| pubmed-article:3545823 | lifeskim:mentions | umls-concept:C1522605 | lld:lifeskim |
| pubmed-article:3545823 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3545823 | pubmed:dateCreated | 1987-4-20 | lld:pubmed |
| pubmed-article:3545823 | pubmed:abstractText | RNA polymerase was treated in the presence of promoter-containing templates with 16 affinity reagents, derivatives on NMPs, NDPs and NTPs with reactive substituents at the terminal phosphate. This treatment was followed by addition of a pyrimidine [alpha-32P]NTP. Due to 'catalytic competence' of some of the residues of the affinity reagents bound covalently near the active center at the first stage, active-center-catalyzed synthesis of a phosphodiester bond occurred, and radioactive residues with the general formula -pNpN (where p = radioactive phosphate) appeared covalently attached to the enzyme. Such affinity labelling was super-selective because affinity reagent residues bound outside the active center were not elongated and thus remained non-radioactive. Labelling took place only when the combination of the reagent and [alpha-32P]NTP corresponded to the sequence of nucleotides of the promoter. With reagents having short 'arms', only the beta subunit was labelled; the targets were His and/or Lys residues. With reagents having longer 'arms', the sigma subunit was also labelled. | lld:pubmed |
| pubmed-article:3545823 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3545823 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3545823 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3545823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3545823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3545823 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3545823 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:3545823 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:3545823 | pubmed:author | pubmed-author:GrachevM AMA | lld:pubmed |
| pubmed-article:3545823 | pubmed:author | pubmed-author:KolochevaT... | lld:pubmed |
| pubmed-article:3545823 | pubmed:author | pubmed-author:MustaevA AAA | lld:pubmed |
| pubmed-article:3545823 | pubmed:author | pubmed-author:LukhtanovE... | lld:pubmed |
| pubmed-article:3545823 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3545823 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:3545823 | pubmed:volume | 163 | lld:pubmed |
| pubmed-article:3545823 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3545823 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3545823 | pubmed:pagination | 113-21 | lld:pubmed |
| pubmed-article:3545823 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
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| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
| pubmed-article:3545823 | pubmed:meshHeading | pubmed-meshheading:3545823-... | lld:pubmed |
| pubmed-article:3545823 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3545823 | pubmed:articleTitle | Studies on the functional topography of Escherichia coli RNA polymerase. Highly selective affinity labelling by analogues of initiating substrates. | lld:pubmed |
| pubmed-article:3545823 | pubmed:publicationType | Journal Article | lld:pubmed |
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