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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-3-6
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pubmed:abstractText |
Bone marrow cells (BMC) flushed from femora of Lewis rats were cultured in Dulbecco's modification of Eagle's medium supplemented with mouse L929 cell supernatant as a source of colony-stimulating factor (CSF). Differentiation of macrophage progenitor cells into macrophages (M phi) and expression of various markers were kinetically assessed. The proportion of M phi increases from approximately 4% in freshly isolated BMC to 100% after 7-8 days of cell culture. These cells, termed bone marrow cell-derived macrophages (BMDM phi), adhere to and spread on plastic surface; exhibit M phi morphology; stain intensely for nonspecific esterase; are able to phagocytose latex particles, IgG-sensitized erythrocytes, and C3-coated red cells; and express receptors for IgG and C3. A subpopulation of BMDM phi expresses MHC class II antigens as demonstrated by immunofluorescence using MRC OX6 and MRC OX17 monoclonal antibodies which recognize antigens coded in the I-A or I-E subregion of the MHC, respectively. Collectively, our results show that supernatant from mouse L929 cells supports and is continuously required for proliferation and differentiation of rat BMC into typical M phi, and suggest that mouse CSF cross-reacts with the putative receptor on rat M phi.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colony-Stimulating Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Complement,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0741-5400
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
83-91
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3543182-Animals,
pubmed-meshheading:3543182-Bone Marrow Cells,
pubmed-meshheading:3543182-Cell Differentiation,
pubmed-meshheading:3543182-Cell Division,
pubmed-meshheading:3543182-Colony-Stimulating Factors,
pubmed-meshheading:3543182-Histocompatibility Antigens Class II,
pubmed-meshheading:3543182-Histocytochemistry,
pubmed-meshheading:3543182-Macrophage-1 Antigen,
pubmed-meshheading:3543182-Macrophages,
pubmed-meshheading:3543182-Male,
pubmed-meshheading:3543182-Mice,
pubmed-meshheading:3543182-Phagocytosis,
pubmed-meshheading:3543182-Rats,
pubmed-meshheading:3543182-Rats, Inbred Lew,
pubmed-meshheading:3543182-Receptors, Complement,
pubmed-meshheading:3543182-Receptors, Fc,
pubmed-meshheading:3543182-Species Specificity
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pubmed:year |
1987
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pubmed:articleTitle |
Differentiation of rat bone marrow cells into macrophages under the influence of mouse L929 cell supernatant.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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