Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1987-1-23
|
| pubmed:abstractText |
The construction of exon-specific HLA-DR beta cDNA clones and their application as DNA or RNA hybridization probes in DR allogenotyping are described. Using a probe derived from the beta 2, transmembrane, cytoplasmic and 3'-untranslated exons of the DR beta chain gene (pRTV1), it is possible to discriminate between DR alleles using the restriction endonucleases TaqI or BamHI. Micro-scale chromosomal DNA isolation procedures, used in conjunction with Southern blot analysis in minigels, facilitate the rapid identification of DR allogenotypes without significant loss of hybridization signal resolution.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0161-5890
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1111-6
|
| pubmed:dateRevised |
2000-12-18
|
| pubmed:meshHeading |
pubmed-meshheading:3540616-Alleles,
pubmed-meshheading:3540616-Cloning, Molecular,
pubmed-meshheading:3540616-DNA,
pubmed-meshheading:3540616-Electrophoresis, Agar Gel,
pubmed-meshheading:3540616-Exons,
pubmed-meshheading:3540616-Genotype,
pubmed-meshheading:3540616-HLA-D Antigens,
pubmed-meshheading:3540616-HLA-DR Antigens,
pubmed-meshheading:3540616-Immunologic Techniques,
pubmed-meshheading:3540616-Molecular Weight,
pubmed-meshheading:3540616-Nucleic Acid Hybridization
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
HLA-DR allogenotyping using exon-specific cDNA probes and application of rapid minigel methods.
|
| pubmed:publicationType |
Journal Article
|