Linked Life Data

3533062

Source:http://linkedlifedata.com/resource/pubmed/id/3533062

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-10-31
pubmed:abstractText
A unique Vero cell cytotoxin has been purified to homogeneity from a strain of Escherichia coli O157:H7, using ultrafiltration with Pellicon membrane cassettes and chromatography with QAE Sephadex A-50. SDS-PAGE showed the molecular weight of the toxin to be 64,000 and the absence of subunits. Based on analytical isoelectric focusing, the toxin had pI of 5.2. This Vero cell toxin was lethal to mice and showed pathological abnormalities of the mouse colonic mucosa when administered intraperitoneally. Vero cell cytotoxicity of this toxin was not neutralizable with rabbit antiserum to Shiga toxin. Based on physicochemical and immunological properties, this toxin is different from the Shiga-like toxin previously found in this organism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
424-30
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Purification and physicochemical properties of a unique Vero cell cytotoxin from Escherichia coli O157:H7.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't