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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1986-10-2
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pubmed:abstractText |
The present investigation introduces a method for purification of human epidermal Langerhans cells (LC). The method is based on the attachment of LC to IgG-coated sheep erythrocyte monolayers via their Fc receptors. To optimize the enrichment assay, several variables were tested. The best results were obtained when epidermal cells were centrifuged against erythrocyte monolayers; the purification procedure was performed at 4 degrees C in the presence of 5% fetal calf serum, using about 6 X 10(6) epidermal cells per erythrocyte plate (diameter 5 cm). The average purity of the recovered LC was 80.9% and LC-depleted fractions contained an average of 0.5% DR-positive cells. LC were able to enhance significantly leukoagglutinin- and purified protein derivative-induced T lymphocyte proliferation and leukocyte migration inhibitory factor production.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0022-202X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3528306-Animals,
pubmed-meshheading:3528306-Cell Adhesion,
pubmed-meshheading:3528306-Cell Separation,
pubmed-meshheading:3528306-Cells, Cultured,
pubmed-meshheading:3528306-Erythrocytes,
pubmed-meshheading:3528306-Humans,
pubmed-meshheading:3528306-Immunoglobulin G,
pubmed-meshheading:3528306-Langerhans Cells,
pubmed-meshheading:3528306-Leukocyte Migration-Inhibitory Factors,
pubmed-meshheading:3528306-Lymphocyte Activation,
pubmed-meshheading:3528306-Receptors, Fc,
pubmed-meshheading:3528306-Sheep,
pubmed-meshheading:3528306-T-Lymphocytes
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pubmed:year |
1986
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pubmed:articleTitle |
Enrichment of human epidermal Langerhans cells by attachment to erythrocyte monolayers.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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