Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-10-15
pubmed:abstractText
EMS-induced mutations within a 180 base pair region of the lacI gene of E. coli were cloned and sequenced. In total, 105 and 79 EMS-induced mutations from a Uvr+ and a UvrB- strain, respectively, were sequenced. The specificity of EMS-induced mutagenesis was very similar in the two strains; G:C----A:T transitions accounted for all but three of the mutants. The overall frequency of induced mutation was fivefold higher in the UvrB- strain compared to the Uvr+ strain. This demonstrates, at the DNA sequence level, that the presumed premutagenic lesion, O6-ethylguanine, is subject to repair by the uvrABC excision repair system of E. coli. An analysis of mutation frequencies with respect to neighboring base sequence, in the two strains, shows that O6-ethylguanine lesions adjacent to A:T base pairs present better targets for the excision repair machinery than those not adjacent to A:T base pairs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-325420, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-416218, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-4355598, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-6093094, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-6348776, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-6370685, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-6392567, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-6865996, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-6866769, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-7022177, http://linkedlifedata.com/resource/pubmed/commentcorrection/3527868-7354843
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
811-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
DNA sequence analysis of mutagenicity and site specificity of ethyl methanesulfonate in Uvr+ and UvrB- strains of Escherichia coli.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't