Linked Life Data

3523063

Source:http://linkedlifedata.com/resource/pubmed/id/3523063

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-8-13
pubmed:abstractText
The effect of age on calcium release in organ culture was studied using calvaria from 5-, 14-, 30-, and 75-day-old mice. The results demonstrated a different and characteristic calcium release pattern over the 14-day culture period for untreated calvaria of different ages. Since calcium release in all cultures was abolished by procedures such as boiling, multiple freezing and thawing, maintaining cultures in an oxygen-free gas phase, or maintaining cultures in a non-nutritive medium, it was concluded that the calcium release from older calvaria was due to a cell-mediated process. Histological observations demonstrating the presence of Howship's lacunae and active osteoclastic resorption confirmed that calcium release from older calvaria was due to an active bone resorption process. Parathyroid hormone did not substantially alter the general pattern of calcium release exhibited by different aged calvaria. However, it tended to exaggerate the magnitude of the response. Indomethacin and dexamethasone inhibited calcium release from untreated 5- and 75-day-old calvaria suggesting that prostaglandin biosynthesis was involved in the calcium release process. Direct measurements of PGE2 and PGI2 released into the culture medium gave results consistent with this hypothesis, although it is conceivable that indomethacin and dexamethasone might have influenced calcium release by other mechanisms.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0047-6374
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-88
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Effect of age on calcium release from mouse calvaria in tissue culture.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't