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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1986-8-5
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pubmed:abstractText |
A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The characteristic morphology of neurons is maintained, together with the diversity of active channels evident in the intact nervous system.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0165-0270
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
227-38
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3523050-Aging,
pubmed-meshheading:3523050-Animals,
pubmed-meshheading:3523050-Brain,
pubmed-meshheading:3523050-Cell Separation,
pubmed-meshheading:3523050-Cell Survival,
pubmed-meshheading:3523050-Electric Stimulation,
pubmed-meshheading:3523050-Guinea Pigs,
pubmed-meshheading:3523050-Membrane Potentials,
pubmed-meshheading:3523050-Microscopy, Electron, Scanning,
pubmed-meshheading:3523050-Microscopy, Phase-Contrast,
pubmed-meshheading:3523050-Neurons
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pubmed:year |
1986
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pubmed:articleTitle |
Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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