Linked Life Data

3523018

Source:http://linkedlifedata.com/resource/pubmed/id/3523018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-8-11
pubmed:abstractText
A sequential corneal endothelium staining technique with trypan blue (TB)--alizarin red S (ARS)--ethanol-trypan blue is described which can be performed in a few minutes. The first step permits the TB exclusion to be determined as a criterion of normal endothelial cell function. The two following steps permit intercellular staining by ARS and nuclear staining by TB. After each staining procedure, fixation can be performed by methanol or ethanol, both with full-thickness corneal preparations or endothelial flat mounts. In flat preparations, the focussing problems and optical artifacts of full-thickness mounts can be avoided. These modifications render simple sequential staining with subsequent fixation a complement and alternative to specular microscopy and to the customary complicated silver staining of corneal endothelium.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0023-2165
pubmed:author
pubmed:issnType
Print
pubmed:volume
188
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
302-6
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
[Trypan blue and alizarin red S sequential staining with subsequent fixation. A modified technic for the study of the corneal endothelium].
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't