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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1986-7-30
|
| pubmed:abstractText |
The lysis by human and murine anti-HLA cytolytic T lymphocytes (CTL) of murine cells expressing class I HLA molecule after gene transfection has been studied using two different murine cells: LMTK- and P815-HTR-TK-. Weak but significant HLA-A11-specific lysis was found occasionally with human CTL on the HLA-A11+ L cells. On the contrary, P815-A11 or P815-A2 cells were lysed strongly and specifically by HLA-A11 or HLA-A2-specific human CTL. The T8+T4- phenotype of the effector cells was confirmed and the reaction was inhibited by anti-HLA class I monoclonal antibodies. Despite their higher sensitivity to human CTL, the P815-HLA+ cells did not express higher levels of HLA antigens than L cells, and the presence or the absence of human beta 2 microglobulin was irrelevant. Anti-human LFA-1 antibodies abrogated the lysis of P815-A11+ cells showing that the LFA-1 receptor which is apparently lacking on the L cell surface was on the contrary expressed on P815 cells. On the other hand, murine anti-HLA CTL have been prepared by immunizing mice against syngeneic HLA-A11+ L cells. They lysed very efficiently and specifically these cells, but appeared completely devoid of activity against human HLA-A11 target cells. This barrier was apparently due to the H-2 restriction of these H-2k anti-HLA murine CTL, as shown by their inability to lyse allogeneic H-2d cells expressing HLA-A11, and by the blocking of their activity by anti H-2k antibodies. By contrast, xenogeneic anti-HLA CTL obtained by immunizing murine lymphocytes against human cells lysed both human and murine HLA+ cells but they reacted with a monomorphic epitope of the HLA molecule in a nonrestricted way. These results show that human cells lyse very efficiently P815 murine cells expressing HLA class I antigens; the higher sensitivity of P815 cells compared to L cells is probably due to the presence of a LFA-1 receptor on these cells; a class I molecule of human origin can be seen as an H-2-restricted minor histocompatibility antigen in another species.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A11 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
597-604
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3522244-Animals,
pubmed-meshheading:3522244-Antigens, Surface,
pubmed-meshheading:3522244-Binding, Competitive,
pubmed-meshheading:3522244-Cell Line,
pubmed-meshheading:3522244-Cytotoxicity, Immunologic,
pubmed-meshheading:3522244-Dose-Response Relationship, Immunologic,
pubmed-meshheading:3522244-H-2 Antigens,
pubmed-meshheading:3522244-HLA Antigens,
pubmed-meshheading:3522244-HLA-A Antigens,
pubmed-meshheading:3522244-HLA-A11 Antigen,
pubmed-meshheading:3522244-Humans,
pubmed-meshheading:3522244-L Cells (Cell Line),
pubmed-meshheading:3522244-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:3522244-Mice,
pubmed-meshheading:3522244-Mice, Inbred C3H,
pubmed-meshheading:3522244-Mice, Inbred DBA,
pubmed-meshheading:3522244-Species Specificity,
pubmed-meshheading:3522244-T-Lymphocytes, Cytotoxic
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Specific lysis of murine cells expressing HLA molecules by allospecific human and murine H-2-restricted anti-HLA T killer lymphocytes.
|
| pubmed:publicationType |
Journal Article
|