| pubmed-article:3520936 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C0016057 | lld:lifeskim |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C0032145 | lld:lifeskim |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C0026385 | lld:lifeskim |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C1446680 | lld:lifeskim |
| pubmed-article:3520936 | lifeskim:mentions | umls-concept:C0008551 | lld:lifeskim |
| pubmed-article:3520936 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:3520936 | pubmed:dateCreated | 1986-7-14 | lld:pubmed |
| pubmed-article:3520936 | pubmed:abstractText | Mouse monoclonal antibodies were derived against a plasminogen activator inhibitor with a mol. wt. of approximately 54,000 (54 K) from the human fibrosarcoma cell line HT-1080. Screening for hybrids producing antibodies directed against the inhibitor was performed with enzyme-linked immunosorbent assay and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting. Four clones of hybridomas producing IgG1 antibodies were further characterized. The inhibitor was purified approximately 50-fold to homogeneity from conditioned cell culture fluid with a yield of approximately 85% by a one-step procedure using Sepharose-conjugated monoclonal antibody. In the 125I-fibrin plate assay one of the antibodies neutralized the effect of the inhibitor on urokinase-type plasminogen activator. Two of the antibodies bound complexes between urokinase-type plasminogen activator and inhibitor while the remaining two antibodies did not. The antibodies could be used for immunocytochemical localization of the inhibitor in HT-1080 cells. All four antibodies cross-reacted with a plasminogen activator inhibitor derived from cultured human umbilical cord endothelial cells. | lld:pubmed |
| pubmed-article:3520936 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3520936 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3520936 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3520936 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3520936 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3520936 | pubmed:issn | 0340-6245 | lld:pubmed |
| pubmed-article:3520936 | pubmed:author | pubmed-author:NielsenL SLS | lld:pubmed |
| pubmed-article:3520936 | pubmed:author | pubmed-author:KristensenPP | lld:pubmed |
| pubmed-article:3520936 | pubmed:author | pubmed-author:AndreasenP... | lld:pubmed |
| pubmed-article:3520936 | pubmed:author | pubmed-author:DanøKK | lld:pubmed |
| pubmed-article:3520936 | pubmed:author | pubmed-author:HuangJ YJY | lld:pubmed |
| pubmed-article:3520936 | pubmed:author | pubmed-author:Grøndahl-Hans... | lld:pubmed |
| pubmed-article:3520936 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3520936 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:3520936 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:3520936 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3520936 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3520936 | pubmed:pagination | 206-12 | lld:pubmed |
| pubmed-article:3520936 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:3520936 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3520936 | pubmed:articleTitle | Monoclonal antibodies to human 54,000 molecular weight plasminogen activator inhibitor from fibrosarcoma cells--inhibitor neutralization and one-step affinity purification. | lld:pubmed |
| pubmed-article:3520936 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3520936 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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