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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1986-7-14
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pubmed:abstractText |
Mouse monoclonal antibodies were derived against a plasminogen activator inhibitor with a mol. wt. of approximately 54,000 (54 K) from the human fibrosarcoma cell line HT-1080. Screening for hybrids producing antibodies directed against the inhibitor was performed with enzyme-linked immunosorbent assay and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting. Four clones of hybridomas producing IgG1 antibodies were further characterized. The inhibitor was purified approximately 50-fold to homogeneity from conditioned cell culture fluid with a yield of approximately 85% by a one-step procedure using Sepharose-conjugated monoclonal antibody. In the 125I-fibrin plate assay one of the antibodies neutralized the effect of the inhibitor on urokinase-type plasminogen activator. Two of the antibodies bound complexes between urokinase-type plasminogen activator and inhibitor while the remaining two antibodies did not. The antibodies could be used for immunocytochemical localization of the inhibitor in HT-1080 cells. All four antibodies cross-reacted with a plasminogen activator inhibitor derived from cultured human umbilical cord endothelial cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0340-6245
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
206-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3520936-Animals,
pubmed-meshheading:3520936-Antibodies, Monoclonal,
pubmed-meshheading:3520936-Cell Line,
pubmed-meshheading:3520936-Chromatography, Affinity,
pubmed-meshheading:3520936-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3520936-Endothelium,
pubmed-meshheading:3520936-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3520936-Fibrosarcoma,
pubmed-meshheading:3520936-Glycoproteins,
pubmed-meshheading:3520936-Histocytochemistry,
pubmed-meshheading:3520936-Humans,
pubmed-meshheading:3520936-Hybridomas,
pubmed-meshheading:3520936-Immunoenzyme Techniques,
pubmed-meshheading:3520936-Immunoglobulin G,
pubmed-meshheading:3520936-Mice,
pubmed-meshheading:3520936-Mice, Inbred BALB C,
pubmed-meshheading:3520936-Molecular Weight,
pubmed-meshheading:3520936-Neutralization Tests,
pubmed-meshheading:3520936-Plasminogen Activators,
pubmed-meshheading:3520936-Plasminogen Inactivators,
pubmed-meshheading:3520936-Umbilical Cord,
pubmed-meshheading:3520936-Urokinase-Type Plasminogen Activator
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pubmed:year |
1986
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pubmed:articleTitle |
Monoclonal antibodies to human 54,000 molecular weight plasminogen activator inhibitor from fibrosarcoma cells--inhibitor neutralization and one-step affinity purification.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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