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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1986-7-9
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pubmed:abstractText |
The development of highly specific antisera to human interleukin 1 (IL-1) has been an elusive goal hampered mainly by the availability of only limited amounts of pure immunogen. To surmount this difficulty, three peptides of the major charged species of IL-1 (pI 6.8) were synthesized and covalently coupled to keyhole limpet hemocyanin (KLH). All three peptide-KLH conjugates raised rabbit heterologous antisera that bound intact pure IL-1 in a dose-dependent and domain-specific manner. Immunoblot analysis of crude concentrated culture supernatants with these antisera showed each of them to be highly specific for mature 18-kDa IL-1. Immunoblot analysis of monocyte lysates revealed a single 33-kDa band consistent with the size of the IL-1 precursor molecule deduced from cloned cDNA. These reagents should prove to be valuable tools in the localization and measurement of IL-1 in cells and fluids and may permit the separate study of individual IL-1 species as well as discrete domains of intact IL-1 molecules.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-22079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-2578046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-2933048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-2989698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-2991600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-3875682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6083565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6190920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6209582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6325504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6332170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6460819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6609981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6611371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-6828080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3520562-939761
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3972-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3520562-Animals,
pubmed-meshheading:3520562-Antibody Specificity,
pubmed-meshheading:3520562-Humans,
pubmed-meshheading:3520562-Immunosorbent Techniques,
pubmed-meshheading:3520562-Interleukin-1,
pubmed-meshheading:3520562-Isoelectric Point,
pubmed-meshheading:3520562-Molecular Weight,
pubmed-meshheading:3520562-Peptide Fragments,
pubmed-meshheading:3520562-Protein Precursors,
pubmed-meshheading:3520562-Rabbits,
pubmed-meshheading:3520562-Radioimmunoassay
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pubmed:year |
1986
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pubmed:articleTitle |
Antibodies of predetermined specificity to the major charged species of human interleukin 1.
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pubmed:publicationType |
Journal Article
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