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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1986-7-2
|
| pubmed:abstractText |
Whereas collecting duct epithelium in vivo is composed of principal and intercalated cells, we grew a pure principal cell epithelium using a new technique involving tissue culture. These principal cells were derived from collecting duct anlagen of newborn rabbits. We investigated the electrical properties of such epithelia in a newly designed lucite double-chamber with an inner opening of 0.08 cm2. Our observations were: mean transepithelial resistance Rte was 0.83 +/- 0.2 k omega cm2 at 37 degrees C and after preincubation in aldosterone; mean transepithelial potential difference Vte was low and variable under standard conditions and at room temperature but increased to -59.5 +/- 4.4 mV (sign referring to polarity of apical surface) after preincubation in 10(-6) mol/l aldosterone and at 37 degrees C; 10(-6) mol/l amiloride added to the apical perfusion fluid largely abolished this Vte while increasing Rte by 120%; experiments with 5 X 10(-3) mol/l BaCl2 in the apical perfusion fluid failed to change Rte and Vte significantly. This principal cell epithelium therefore has characteristics of a "tight" epithelium with active sodium transport; however, its electrical properties differ from those of the isolated perfused collecting duct segment.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aldosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Barium,
http://linkedlifedata.com/resource/pubmed/chemical/Barium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/barium chloride
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
406
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
380-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3520475-Aldosterone,
pubmed-meshheading:3520475-Amiloride,
pubmed-meshheading:3520475-Animals,
pubmed-meshheading:3520475-Barium,
pubmed-meshheading:3520475-Barium Compounds,
pubmed-meshheading:3520475-Biological Transport,
pubmed-meshheading:3520475-Cell Membrane,
pubmed-meshheading:3520475-Chlorides,
pubmed-meshheading:3520475-Culture Techniques,
pubmed-meshheading:3520475-Electrophysiology,
pubmed-meshheading:3520475-Epithelial Cells,
pubmed-meshheading:3520475-Epithelium,
pubmed-meshheading:3520475-Freeze Fracturing,
pubmed-meshheading:3520475-Histological Techniques,
pubmed-meshheading:3520475-Kidney Tubules,
pubmed-meshheading:3520475-Kidney Tubules, Collecting,
pubmed-meshheading:3520475-Microscopy, Electron,
pubmed-meshheading:3520475-Rabbits,
pubmed-meshheading:3520475-Sodium
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Electrical properties of renal collecting duct principal cell epithelium in tissue culture.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|