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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-6-10
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pubmed:abstractText |
Lipopolysaccharide extracted with phenol-water from Leptospira interrogans serovar copenhageni strain Shibaura (L-LPS) showed various biological activities. In lethality for mice, L-LPS was active (LD 50, 3.4 mg/mouse) but about 12 times less potent than Escherichia coli LPS (E-LPS) per weight basis. L-LPS had pyrogenicity for rabbits, and the fever curves showed no evidence of the classical biphasic fever produced by E-LPS. In the bone marrow of mice, L-LPS caused hemorrhages and necrosis but less severe than those caused by E-LPS. Histopathologically, fresh hemorrhages were found in the intestine, spleen, lung and the other organs at 24 h after inoculation of L-LPS. Necrosis was also found in these organs and was particularly severe in mice inoculated with more than 2 mgL-LPS. Liver necrosis was found at 7th day after inoculation of L-LPS but not after inoculation of E-LPS. L-LPS had adjuvant activity just like E-LPS. L-LPS enhanced non-specific resistance to Salmonella infection and activated mouse peritoneal macrophages to kill these organisms. L-LPS was positive in limulus test just like E-LPS. These results demonstrated similarities of L-LPS and E-LPS. Some toxic effects of L-LPS were less than those of E-LPS, but some effects of L-LPS were more than those of E-LPS. L-LPS was antigenically active and the specificity was serogroup-associated. L-LPS was composed of carbohydrate (54%), lipid (12%), protein (5%). Arabinose, xylose and rhamnose were major sugars as detected by gas chromatography. 2-keto-deoxyoctanate (KDO) was not detectable.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0176-6724
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
261
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-64
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pubmed:dateRevised |
2009-6-8
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pubmed:meshHeading |
pubmed-meshheading:3518294-Animals,
pubmed-meshheading:3518294-Bone Marrow,
pubmed-meshheading:3518294-Escherichia coli,
pubmed-meshheading:3518294-Fever,
pubmed-meshheading:3518294-Hemorrhage,
pubmed-meshheading:3518294-Immunity, Innate,
pubmed-meshheading:3518294-Intestines,
pubmed-meshheading:3518294-Kidney,
pubmed-meshheading:3518294-Leptospira interrogans,
pubmed-meshheading:3518294-Lipopolysaccharides,
pubmed-meshheading:3518294-Lung,
pubmed-meshheading:3518294-Macrophage Activation,
pubmed-meshheading:3518294-Macrophages,
pubmed-meshheading:3518294-Male,
pubmed-meshheading:3518294-Mice,
pubmed-meshheading:3518294-Necrosis,
pubmed-meshheading:3518294-Phagocytosis,
pubmed-meshheading:3518294-Rabbits,
pubmed-meshheading:3518294-Salmonella Infections, Animal,
pubmed-meshheading:3518294-Spleen
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pubmed:year |
1986
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pubmed:articleTitle |
Biological activities of leptospiral lipopolysaccharide.
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pubmed:publicationType |
Journal Article,
Comparative Study
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