Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1986-5-23
pubmed:abstractText
The hypothesis is tested that the ras gene of Harvey sarcoma virus (Ha-SV) and the proto-ras DNAs from certain tumor cells derive transforming function from specific codons in which they differ from normal proto-ras genes. Molecularly cloned Harvey proviral vectors carrying viral ras, normal rat proto-ras, and recombinant ras genes in which the virus-specific ras codons 12 and 59 were replaced by proto-ras equivalents each transformed aneuploid mouse 3T3 cells after latent periods that ranged from 4 to 10 days. Viruses with or without virus-specific ras codons all transformed diploid rat cells in 3-5 days equally well. However, in the absence of virus replication, mutant codons were beneficial for transforming function. Deletion of non-ras regions of Ha-SV did not affect transforming function. We conclude that specific ras codons are not necessary for transforming function. Comparisons of the ras sequences of Ha-SV, BALB SV, and Rasheed SV with sequences of proto-ras genes from rat and man revealed an upstream proto-ras exon, termed exon -1. The 3' end of this exon is present in all three viruses and in a ras pseudogene of the rat. Since ras genes transform without mutation and since exon -1 is truncated in viral ras genes and all transforming proto-ras DNAs of the Harvey and the Kirsten ras family, we propose that ras genes are activated by truncation of exon -1 either via viral transduction or artificially via cloning and transfection. The proposal implies that untruncated proto-ras genes with point mutations may not be cellular cancer genes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-163009, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-221684, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2981345, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2983103, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2983346, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2986131, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2987541, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2989702, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2996222, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-2999983, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-3510078, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-3876558, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-3992240, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-4312364, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-4333859, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-4357879, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-4737663, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6092966, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6097027, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6131379, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6253666, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6261656, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6267479, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6267583, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6283357, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6283358, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6286138, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6287573, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6290897, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6298635, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6308466, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6308467, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6318112, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6327295, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6330729, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6344220, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6397126, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6457158, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6462235, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6694757, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6700714, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6740333, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6844927, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-6953433, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-7063039, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-7133135, http://linkedlifedata.com/resource/pubmed/commentcorrection/3517865-7177195
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2340-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Harvey ras genes transform without mutant codons, apparently activated by truncation of a 5' exon (exon -1).
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't