Linked Life Data

3516471

Source:http://linkedlifedata.com/resource/pubmed/id/3516471

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-6-18
pubmed:abstractText
Leu-M2 (Mac-120) is a mouse anti-human monoclonal antibody that reacts with an antigen on the surface of most peripheral blood monocytes. The presence of Leu-M2 positivity has been associated with the capacity of monocytes to present antigen to T cells. We found that anti-Leu-M2 does not react with an intrinsic monocyte antigen but instead binds to platelets present on the surface of monocytes. Leu-M2 staining is lost from monocytes after they are treated with calcium chelating agents which remove platelets bound to the monocyte surface. Staining for Leu-M2 can be reintroduced by incubating monocytes with autologous platelets. Human alveolar, peritoneal, and tissue macrophages and the U937 cell failed to stain for Leu-M2 antigen. Leu-M2 does not appear to represent either platelet glycoprotein IIb/IIIa or thrombospondin. Our study underscores the importance of carefully screening antimonocyte antibodies for their reactivity with platelets.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0090-1229
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
442-51
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Leu-M2 (Mac-120) is a platelet antigen and is not constitutively expressed by macrophages.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.