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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-5-16
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pubmed:abstractText |
A convenient procedure for the synthesis and purification of oligonucleotides is described. 16-base long primers synthesised by this method were used to investigate DNA sequencing using plasmid DNA as a template. This allowed the further analysis of the E. coli glt A sequence coding for citrate synthase and enabled determination of the 5'-non-coding regulatory region of the aminoglycoside phosphotransferase gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:issn |
0020-711X
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
257-62
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:3514304-Base Sequence,
pubmed-meshheading:3514304-Coliphages,
pubmed-meshheading:3514304-DNA, Bacterial,
pubmed-meshheading:3514304-DNA, Viral,
pubmed-meshheading:3514304-Escherichia coli,
pubmed-meshheading:3514304-Indicators and Reagents,
pubmed-meshheading:3514304-Oligodeoxyribonucleotides,
pubmed-meshheading:3514304-Plasmids,
pubmed-meshheading:3514304-Templates, Genetic
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|
pubmed:year |
1986
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|
pubmed:articleTitle |
The synthesis and use of oligodeoxynucleotides in plasmid DNA sequencing.
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pubmed:publicationType |
Journal Article
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