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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1986-4-24
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pubmed:databankReference |
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pubmed:abstractText |
We have investigated the molecular basis for a 25- to 30-fold overexpression of the c-Ki-ras oncogene in a mouse bone marrow-derived, early myeloid cell line, 416B. Southern blot hybridizations revealed that the 416B cells contain a rearranged c-Ki-ras gene in addition to an apparently normal gene. Molecular cloning and DNA sequence analyses demonstrated that the rearrangement involves the insertion of a 3.5-kilobase-pair segment of Friend virus that includes the envelope gene (env) and 3' long terminal repeat. The Friend provirus is positioned between a 5' nontranslated exon (exon phi) and the first coding exon (exon 1) of the c-Ki-ras gene in the same transcriptional orientation. Results of RNA blot analyses indicate that transcription from the rearranged gene initiates at a promoter that excludes sequences in exon phi. The data support the hypothesis that enhanced c-Ki-ras expression in the 416B cells results from integration of a Friend provirus within this gene.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-2992942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-4006517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-4006913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6087294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6092920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6098468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6131379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6245244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6258798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6261142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6265801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6276760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6283357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6283358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6285355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6295880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6296423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6297757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6304530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6305505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6305506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6308465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6308467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6308661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6312107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6315951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6318122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6320174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6321768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6322173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6325920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6326104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6327047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6327049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6327073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6474169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6591200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6595484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6695174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-6821513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-7144906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-7237541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3513183-763330
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1651-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3513183-Animals,
pubmed-meshheading:3513183-Base Sequence,
pubmed-meshheading:3513183-Bone Marrow Cells,
pubmed-meshheading:3513183-Cell Line,
pubmed-meshheading:3513183-Cell Transformation, Viral,
pubmed-meshheading:3513183-Cloning, Molecular,
pubmed-meshheading:3513183-Female,
pubmed-meshheading:3513183-Friend murine leukemia virus,
pubmed-meshheading:3513183-Gene Expression Regulation,
pubmed-meshheading:3513183-Mice,
pubmed-meshheading:3513183-Promoter Regions, Genetic,
pubmed-meshheading:3513183-Proto-Oncogene Proteins,
pubmed-meshheading:3513183-Proto-Oncogenes,
pubmed-meshheading:3513183-RNA, Messenger,
pubmed-meshheading:3513183-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:3513183-Transcription, Genetic,
pubmed-meshheading:3513183-Viral Envelope Proteins
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pubmed:year |
1986
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pubmed:articleTitle |
Enhanced c-Ki-ras expression associated with Friend virus integration in a bone marrow-derived mouse cell line.
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pubmed:publicationType |
Journal Article
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