Linked Life Data

3512718

Source:http://linkedlifedata.com/resource/pubmed/id/3512718

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-3-28
pubmed:abstractText
Monoclonal anti-human IgM and anti-rubella antibodies were prepared and tested in an IgM capture enzyme immunoassay (MACEIA) for rubella-specific IgM and compared with polyclonal reagents. Assay sensitivity was increased with monoclonal antibodies resulting in the improved discrimination of adult sera with low levels of specific IgM. Despite high IgM binding, interference by IgM anti-Ig was not a major problem. The use of monoclonal antibodies allowed assay simplification by the simultaneous rather than sequential addition of antigen and conjugate. Although comparable results were obtained with 33 test samples in the sequential and simultaneous MACEIA, the specificity and sensitivity of this modification requires further evaluation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-17
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Selection and performance of monoclonal and polyclonal antibodies in an IgM antibody capture enzyme immunoassay for rubella.
pubmed:publicationType
Journal Article, Comparative Study