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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1986-4-4
|
| pubmed:abstractText |
The neural cell adhesion molecules (N-CAMs) are a group of structurally and immunologically related glycoproteins found in vertebrate neural tissues. Adult brain N-CAMs have apparent molecular weights of 180,000 and 140,000 with an additional form at 120,000 in murine brain. In embryonic brain, N-CAMs are represented by a highly sialylated form with an apparent molecular weight greater than 180,000. We have used monoclonal antibodies that cross-react with N-CAMs of various mammalian species to purify N-CAMs from adult murine and bovine brains and from embryonic murine brains. We determined the amino acid sequences of the amino-terminal domains of these molecules: Leu-Gln-Val-Asp-Ile-Val-Pro-Ser-Gln-Gly-Glu-Ile-Ser-Val-Gly-Glu-Ser. This sequence is highly conserved among all three forms of adult murine brain N-CAM as well as embryonic murine brain N-CAM and adult bovine brain N-CAM. Based on this sequence, we synthesized an undecapeptide and used it to raise a site-directed polyclonal antiserum. This antiserum reacted with the intact N-CAM in liquid phase radioimmunoassays, immunoblotting experiments, and immunofluorescent labeling of cells. The antiserum also reacted with N-CAMs in extracts of brain tissues from different species, confirming the highly conserved nature of the amino-terminal domain of mammalian N-CAMs. Immunofluorescence experiments indicated that this domain resides on the outer surfaces of cells that express N-CAMs, in both primary neuronal cell culture and in cell lines.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
261
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3396-401
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3512556-Amino Acid Sequence,
pubmed-meshheading:3512556-Animals,
pubmed-meshheading:3512556-Antigens, Surface,
pubmed-meshheading:3512556-Brain Chemistry,
pubmed-meshheading:3512556-Cattle,
pubmed-meshheading:3512556-Cell Adhesion Molecules,
pubmed-meshheading:3512556-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3512556-Fluorescent Antibody Technique,
pubmed-meshheading:3512556-Immunosorbent Techniques,
pubmed-meshheading:3512556-Mice,
pubmed-meshheading:3512556-Microscopy, Fluorescence,
pubmed-meshheading:3512556-Molecular Weight
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Structural and immunological characterization of the amino-terminal domain of mammalian neural cell adhesion molecules.
|
| pubmed:publicationType |
Journal Article
|