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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1976-6-2
|
| pubmed:abstractText |
A DNA kinase has been partially purified from rat liver nuclei by a procedure which also yields DNA ligase. The kinase uses ATP to phosphorylate specifically the 5'-hydroxyl termini of oligodeoxynucleotides and of single- or double-stranded DNA, yielding 5'-phosphate termini and ADP. The kinase is inactive on RNA, or on oligodeoxynucleotides of chain length less than approximately 10 to 12 residues. The kinase requires a divalent cation (Mg2+, Mn2+, Co2+, Zn2+, Ni2+, or Ca2+) for activity and has an acidic pH optimum. It is inhibited by a variety of nucleotides as well as by very low levels of inorganic and organic sulfate compounds and sulfate analogues. The molecular weight of the kinase is estimated to be 8 times 10(4) from gel filtration.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
251
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1767-74
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3504-Animals,
pubmed-meshheading:3504-Cations, Divalent,
pubmed-meshheading:3504-Cations, Monovalent,
pubmed-meshheading:3504-Cell Nucleus,
pubmed-meshheading:3504-Hydrogen-Ion Concentration,
pubmed-meshheading:3504-Kinetics,
pubmed-meshheading:3504-Liver,
pubmed-meshheading:3504-Osmolar Concentration,
pubmed-meshheading:3504-Phosphotransferases,
pubmed-meshheading:3504-Polynucleotide 5'-Hydroxyl-Kinase,
pubmed-meshheading:3504-Rats
|
| pubmed:year |
1976
|
| pubmed:articleTitle |
A deoxyribonucleic acid kinase from nuclei of rat liver. Purification and properties.
|
| pubmed:publicationType |
Journal Article
|