Linked Life Data

3503545

Source:http://linkedlifedata.com/resource/pubmed/id/3503545

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-11-14
pubmed:abstractText
Purified chick duodenal brush border membrane vesicles (BBMV) were used to assess the effect of vitamin D on intestinal Ca2+ transport and membrane stability. BBMV preparations are right-side-out as judged by a nine-fold increase in accessibility of lactoperoxidase to core material actin in the presence of Triton X-100. Freshly prepared BBMV from vitamin D-deficient chicks support both sodium-dependent glucose transport and Ca2+ uptake. In vivo treatment with 1,25(OH)2D3 results in an 85% increase in the Vmax of Ca2+-uptake from 2.2 to 3.9 nmol/min/mg protein. The Km of Ca2+-uptake (0.9 mM) is independent of the vitamin D status of the chick. The majority of BBMV derived from vitamin D-replete chicks were destabilized and rendered incapable of supporting either sodium-dependent glucose uptake or Ca2+ uptake if they were held at 0-4 degrees C for 2 to 24 h. In 40 separate experiments, 80% of membranes derived from vitamin D-replete chicks showed characteristics of destabilization, whereas only 24% of all control membranes exhibited a lack of viability.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0884-0431
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-11
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Vitamin D status and brush border membrane vesicles: 1,25-dihydroxyvitamin D3 induced destabilization.
pubmed:affiliation
Department of Biochemistry, University of California, Riverside 92521.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.