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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-3-15
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pubmed:abstractText |
1. In hepatocytes, epidermal growth factor (EFG) (a) increased the rate of 45Ca2+ exchange in cells incubated at 1.3 mM extracellular Ca2+, (b) increased the activity of glycogen phosphorylase a and the intracellular free Ca2+ concentration (measured with quin2) in a process dependent on the concentration of extracellular Ca2+, and (c) enhanced the increase in glycogen phosphorylase activity which follows the addition of Ca2+ to cells previously incubated in the absence of Ca2+. It is concluded that EGF stimulates plasma-membrane Ca2+ inflow. 2. The effects of the combination of EGF and vasopressin on the rate of 45Ca2+ exchange and on the rate of increase in glycogen phosphorylase activity were the same as those of vasopressin alone. 3. The amount of 45Ca2+ released by EGF from internal stores was about 30% of that released by vasopressin. No detectable increase in [3H]inositol mono-, bis- or tris-phosphate was observed after the addition of EGF to cells labelled with myo-[3H]inositol. 4. In hepatocytes isolated from rats treated with pertussis toxin, the effects of EGF and vasopressin on phosphorylase activity (measured at 1.3 mM-Ca2+) and on the rate of Ca2+ inflow (measured with quin2) were markedly decreased compared with those in normal cells. 5. Treatment with pertussis toxin did not impair the ability of vasopressin to release Ca2+ from internal stores, but decreased vasopressin-stimulated [3H]inositol polyphosphate formation by 50%. 6. It is concluded that the mechanism(s) by which vasopressin and EGF stimulate plasma-membrane Ca2+-inflow transporters in hepatocytes involves a GTP-binding regulatory protein sensitive to pertussis toxin, and does not require an increase in the concentration of inositol trisphosphate comparable with that which induces the release of Ca2+ from the endoplasmic reticulum.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2419757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2420465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2428376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2433590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2579078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2860111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2991741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-2999149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3005828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3015910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3030262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3083411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3099775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3117043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3484478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3485384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3486935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3487541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3488233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3827881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3829123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3873238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-3919020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-4016891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-4026798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-4044600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-4084229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-457663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6087338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6087800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6094010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6131669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6272837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6325926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6369317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6477530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6607924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6743271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6743279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6812571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6884512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-6980885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-7264996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-75,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3501716-8456
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
248
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
911-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3501716-Animals,
pubmed-meshheading:3501716-Calcium,
pubmed-meshheading:3501716-Cell Membrane,
pubmed-meshheading:3501716-Cells, Cultured,
pubmed-meshheading:3501716-Enzyme Activation,
pubmed-meshheading:3501716-Epidermal Growth Factor,
pubmed-meshheading:3501716-Inositol Phosphates,
pubmed-meshheading:3501716-Liver,
pubmed-meshheading:3501716-Male,
pubmed-meshheading:3501716-Pertussis Toxin,
pubmed-meshheading:3501716-Phosphorylases,
pubmed-meshheading:3501716-Rats,
pubmed-meshheading:3501716-Rats, Inbred Strains,
pubmed-meshheading:3501716-Vasopressins,
pubmed-meshheading:3501716-Virulence Factors, Bordetella
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pubmed:year |
1987
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pubmed:articleTitle |
Evidence that a pertussis-toxin-sensitive substrate is involved in the stimulation by epidermal growth factor and vasopressin of plasma-membrane Ca2+ inflow in hepatocytes.
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pubmed:affiliation |
Department of Medical Biochemistry, Flinders University School of Medicine, Bedford Park, South Australia.
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