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pubmed-article:3501372pubmed:abstractTextA retrovirus encoding polyoma middle T antigen has been used to infect a murine hemopoietic cell line (FDC-P1) dependent on either granulocyte-macrophage colony-stimulating factor (GM-CSF) or multipotential colony-stimulating factor (Multi-CSF). A number of cell lines have been established on the basis of their initial ability to proliferate in the absence of added colony-stimulating factor (CSF). The transformed lines display one of three patterns of growth in vitro: those able to grow fully autonomously; those whose proliferation depends on cell density; and those displaying dependence on added CSF regardless cell density. This latter class of cells are reminiscent of the majority of primary myeloid leukemic cells. Unlike parental FDC-P1 cells, all three classes of transformed cells are leukemogenic in syngeneic mice; moreover, they produce variable amounts of GM-CSF which we believe underlies their neoplastic behavior.lld:pubmed
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pubmed-article:3501372pubmed:articleTitleThe in vitro behavior of hemopoietic cells transformed by polyoma middle T antigen parallels that of primary human myeloid leukemic cells.lld:pubmed
pubmed-article:3501372pubmed:affiliationWalter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.lld:pubmed
pubmed-article:3501372pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:3501372pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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