Linked Life Data

3500857

Source:http://linkedlifedata.com/resource/pubmed/id/3500857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-2-18
pubmed:abstractText
We have obtained, by transfection of mouse L cells with total human DNA, clones that constitutively secreted human interleukins IL1, IL2 and B-cell grown-factor activities, as assessed by specific biological assays. Southern analysis with IL2 and IL1 beta cDNAs confirmed the integration of the corresponding human genes in the genome of recipient mouse cells and showed their amplification and rearrangement. All the four IL2-secreting clones integrated in the mouse genome the human IL2 gene. Three out of the sixteen IL1-producing clones contained the IL1 beta gene. The IL1 activity secreted by the remaining clones exhibited a molecular mass of 17 kDa similar to that reported for mature human macrophage IL1. Our results demonstrate that DNA-mediated gene transfer may represent a suitable tool for the production of human growth and differentiation factors and the cloning of their genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
669-74
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Expression of interleukins in L cells transfected with human DNA.
pubmed:affiliation
Institute of Biochemical Science, Medical School, University of Naples, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't