Linked Life Data

3498958

Source:http://linkedlifedata.com/resource/pubmed/id/3498958

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1987-11-19
pubmed:abstractText
One goal of our research has been to define the principles necessary to utilize cultured T cells as reagents in vivo in order to augment specific T cell immunity and to utilize the augmented immunity as a form of cancer therapy. A potential barrier for the use of cultured T cells in vivo has been the previously demonstrated inability of cultured T cells to survive in vivo. As an example, studies to be reviewed below showed that a small precursor population of tumor-specific T cells could be grown to large numbers in vitro by repeated supplementation of media with exogenous Interleukin 2 (IL 2) and that the resultant long-term cultured T cells could mediate specific tumor therapy in vivo. However, T cells grown with IL 2 lost the ability to proliferate in response to immune stimulation by tumor antigen, became dependent upon exogenous IL 2 for survival, and thus died rapidly in vivo without repeated administration of exogenous IL 2. By contrast, T cells grown long-term in vitro in response to antigen-stimulation, as opposed to exogenous IL 2, were able to proliferate in vivo in response to stimulation by tumor antigen, mediate tumor therapy and persist long-term in vivo as functional memory T cells. Thus, the previously demonstrated inability of cultured T cells to survive and persist in vivo apparently resulted from the culture conditions utilized and did not reflect an intrinsic defect of all cultured T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0361-7742
pubmed:author
pubmed:issnType
Print
pubmed:volume
244
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-58
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Antigen-specific cultured T cells can mediate tumor therapy and provide long-term immunologic memory in vivo.
pubmed:affiliation
University of Washington, Division of Medical Oncology, Seattle.
pubmed:publicationType
Journal Article