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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0037993,
umls-concept:C0051977,
umls-concept:C0051980,
umls-concept:C0591833,
umls-concept:C0596235,
umls-concept:C0806140,
umls-concept:C0871261,
umls-concept:C0936012,
umls-concept:C1383501,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
4
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pubmed:dateCreated |
1987-10-22
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pubmed:abstractText |
The accurate measurement of ionized intracellular calcium [Ca++]i in single cells by flow cytometry with the use of a new fluorescent calcium chelator, indo-1, is described. We have developed a dependable in situ calibration technique that indicates a resting [Ca++]i in lymphocytes of 100 nM. The enhanced fluorescence of this probe permits its use at sufficiently low cytoplasmic concentrations that buffering of [Ca++]i transients does not occur. The [Ca++]i response of small resting B lymphocytes to crosslinking of surface antigen receptors by anti-immunoglobulin is heterogeneous. With maximal stimulus, the peak [Ca++]i response occurs in 10 to 20 seconds with most cells reaching levels greater than/1 microM. Mean [Ca++]i falls to between 300 and 800 nM by 100 seconds where it remains for more than 10 min. Anti-delta is a more potent stimulus of increased [Ca++]i than anti-mu in terms of both [Ca++]i level and fraction of B cells responding. Whether this is due to the greater density of surface IgD than IgM, a difference in signal transduction efficiency, or both, is not yet known. Surface immunoglobulin receptors are present in great excess. Less than 3% of surface immunoglobulin is crosslinked at the peak of the [Ca++]i response.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin D,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Isotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgD,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgM,
http://linkedlifedata.com/resource/pubmed/chemical/indo-1
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0196-4763
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
396-404
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:3497786-Animals,
pubmed-meshheading:3497786-Antibodies, Anti-Idiotypic,
pubmed-meshheading:3497786-B-Lymphocytes,
pubmed-meshheading:3497786-Calcium,
pubmed-meshheading:3497786-Cytosol,
pubmed-meshheading:3497786-Flow Cytometry,
pubmed-meshheading:3497786-Fluorescent Dyes,
pubmed-meshheading:3497786-Immunoglobulin D,
pubmed-meshheading:3497786-Immunoglobulin Isotypes,
pubmed-meshheading:3497786-Immunoglobulin M,
pubmed-meshheading:3497786-Indoles,
pubmed-meshheading:3497786-Mice,
pubmed-meshheading:3497786-Mice, Inbred BALB C,
pubmed-meshheading:3497786-Receptors, Antigen, B-Cell,
pubmed-meshheading:3497786-Receptors, Immunologic,
pubmed-meshheading:3497786-Spleen
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pubmed:year |
1987
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pubmed:articleTitle |
Flow cytometric analysis of murine splenic B lymphocyte cytosolic free calcium response to anti-IgM and anti-IgD.
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pubmed:publicationType |
Journal Article
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