rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
3
|
pubmed:dateCreated |
1987-8-31
|
pubmed:abstractText |
Isolated rat enterocytes were permeabilized by saponin treatment. 45Ca2+ was accumulated by these cells when provided with ATP in a medium containing Ca2+ ligands. The use of oxalate, vanadate and mitochondrial inhibitors indicated that both non-mitochondrial and mitochondrial pools are involved. Kinetic analysis of non-mitochondrial Ca2+ uptake revealed a Km of 0.1 microM Ca2+ and a Vmax of 0.4 nmol Ca2+/mg protein X min for this Ca2+-pumping ATPase activity. Mitochondria started to take up Ca2+ between 0.2 and 0.3 microM free Ca2+ reaching maximal rates around 2 microM. At 1 microM free Ca2+ mitochondria accumulated 20 times more Ca2+ than the non-mitochondrial pool. Inositol 1,4,5-trisphosphate released 40% of the Ca2+ content of the non-mitochondrial pool. Half-maximal release was observed at 0.5 and 1.5 microM IP3 in duodenal and ileal cells respectively. These findings support the possibility that the phosphatidyl inositide metabolism plays a role in regulation of electrolyte transport in enterocytes.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0143-4160
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
8
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
197-206
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:3496969-Adenosine Triphosphate,
pubmed-meshheading:3496969-Animals,
pubmed-meshheading:3496969-Calcium,
pubmed-meshheading:3496969-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:3496969-Inositol Phosphates,
pubmed-meshheading:3496969-Intestinal Absorption,
pubmed-meshheading:3496969-Intestine, Small,
pubmed-meshheading:3496969-Kinetics,
pubmed-meshheading:3496969-Male,
pubmed-meshheading:3496969-Mitochondria,
pubmed-meshheading:3496969-Rats,
pubmed-meshheading:3496969-Saponins,
pubmed-meshheading:3496969-Sugar Phosphates,
pubmed-meshheading:3496969-Vanadates,
pubmed-meshheading:3496969-Vanadium
|
pubmed:year |
1987
|
pubmed:articleTitle |
Kinetics of ATP-dependent Ca2+ uptake by permeabilized rat enterocytes. Effects of inositol 1,4,5-trisphosphate.
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|