Linked Life Data

3496232

Source:http://linkedlifedata.com/resource/pubmed/id/3496232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1987-8-14
pubmed:abstractText
Filamentous actin has been localized in isolated retinal neurons and glia using fluorescent phallotoxin. Photoreceptors, bipolar cells, horizontal cells, amacrine cells and Müller (glial) cells were isolated by gentle enzymatic digestion of frog, lizard, rabbit, rat and cat retinas. The cells were then fixed in paraformaldehyde and stained with rhodamine-phalloidin. The patterns of fluorescence recorded were specific for each cell type. All interneurons had spots of bright fluorescence along dendrites and (or) axon terminals, probably corresponding to synaptic sites. Horizontal cells and Müller cells had a continuous subplasmalemmal layer of fluorescence throughout; this layer was also present in bipolar cells, but only in the region of cytoplasm at the base of the dendrites. Müller cells also had bright fluorescence in their apical microvilli and terminal web and associated with the zonulae adhaerentes junctions between Müller cells and photoreceptors. All photoreceptors exhibited fluorescence in their synaptic terminals, in a ring just sclerad to the nucleus (corresponding to zonulae adhaerentes junctions formed with Müller cells), and in cables running longitudinally in the inner segment. Frog photoreceptors also had processes alongside their outer segments. Rods from mammals and Xenopus had distinct spots of fluorescence at the outer segment base in a region that suggests involvement with morphogenesis of new outer-segment disc membrane.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-406
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The distribution of F-actin in cells isolated from vertebrate retinas.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.