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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-7-23
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pubmed:abstractText |
Membrane-associated interleukin 1 (IL 1) activity was induced on the human macrophage tumor cell line, U937, by pretreatment with phorbol myristic acid (PMA). Incubation of PMA-treated, paraformaldehyde-fixed U937 cells with the murine cell line D10.G4.1 in the presence of concanavalin A caused an increase in DNA synthesis as measured by the uptake of tritiated thymidine. Paraformaldehyde-fixed U937, not pretreated with PMA, showed little or no activity. A rabbit polyclonal antibody directed against human IL 1 neutralized all membrane-associated IL 1-like activity, as measured by the inhibition of D10.G4.1 cell proliferation. PMA-treated U937 caused a pronounced enhancement of PGE2 production from a human chondrosarcoma cell line, SW-1353. Membrane-associated IL 1 induced a more potent PGE2 response than did a maximal concentration of soluble IL 1. Rabbit antihuman IL 1 neutralized membrane-bound IL 1 induction of PGE2. The data presented here raise the possibility that membrane-bound IL 1 may play a primary role in the pathophysiology of the inflammatory disease process.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
139
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
166-8
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3495597-Cell Membrane,
pubmed-meshheading:3495597-Chondrosarcoma,
pubmed-meshheading:3495597-Dinoprostone,
pubmed-meshheading:3495597-Humans,
pubmed-meshheading:3495597-Interleukin-1,
pubmed-meshheading:3495597-Lymphocyte Activation,
pubmed-meshheading:3495597-Macrophages,
pubmed-meshheading:3495597-Membrane Proteins,
pubmed-meshheading:3495597-Prostaglandins E,
pubmed-meshheading:3495597-T-Lymphocytes
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pubmed:year |
1987
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pubmed:articleTitle |
Membrane-associated interleukin 1 activity on human U937 tumor cells: stimulation of PGE2 production by human chondrosarcoma cells.
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pubmed:publicationType |
Journal Article
|