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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1987-6-9
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pubmed:abstractText |
The present studies characterize at the clonal level the repertoire of lipopolysaccharide-responsive murine B lymphocytes committed to the production of antibodies reactive with denatured DNA. This repertoire is vast in normal mice as 1-5% of total mitogen-induced antibody-forming cell clones secreted denatured DNA-reactive antibodies when the splenocyte donors were CBA (Ighj), BALB/c (Igha), C57BL/6 (Ighb), CBA nu/nu, and C57BL/6 nu/nu athymic mice. The autoimmune NZB (Ighe) strain did not display elevated proportions of anti-denatured DNA antibody-forming cell precursors. Cross-reactions shown by CBA anti-denatured DNA antibodies suggest that many antibodies might derive significant binding energy from interaction with the bases or similar hydrophobic moieties. Cross-reactions with other tested polynucleotides were frequent, but cross-reactions with phospholipids and phosphocholine were undetectable. Most anti-DNA antibodies bound preferentially or exclusively to single-stranded denatured DNA as compared to double-stranded native DNA. The frequency of anti-denatured DNA antibody-forming cell precursors among CBA peritoneal cells was not elevated. Fluorescence-activated cell sorter-selected Ly-1-positive NZB splenic B cells were not enriched, and Ly-1 negative B cells were not depleted of anti-DNA antibody-forming cell precursors. These results show that antibody-forming cell precursors specific for denatured DNA are not restricted to the Ly-1 positive B-cell subset.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-1150882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-2579097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-2580911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-301538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-303258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-304866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3084283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-325169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3333786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3484501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3530507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3876376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3889907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3890479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3891170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-3905971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-4558707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-566858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-57094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6084573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6181395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6189724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6199407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6333448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6399976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6470143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6608423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6609363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6631010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6823251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6972993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-6983913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3495004-70310
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2931-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3495004-Animals,
pubmed-meshheading:3495004-Autoantibodies,
pubmed-meshheading:3495004-B-Lymphocytes,
pubmed-meshheading:3495004-Cells, Cultured,
pubmed-meshheading:3495004-Clone Cells,
pubmed-meshheading:3495004-DNA,
pubmed-meshheading:3495004-Female,
pubmed-meshheading:3495004-Male,
pubmed-meshheading:3495004-Mice,
pubmed-meshheading:3495004-Mice, Inbred Strains,
pubmed-meshheading:3495004-Nucleic Acid Denaturation,
pubmed-meshheading:3495004-Species Specificity
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pubmed:year |
1987
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pubmed:articleTitle |
Clonal analysis of the anti-DNA repertoire of murine B lymphocytes.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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