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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1987-6-5
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pubmed:abstractText |
Native and oxidized alpha 1-proteinase inhibitor (alpha 1-PI) were compared as substrates for the metalloproteinase macrophage elastase. At substrate concentrations at which native alpha 1-PI was readily degraded by macrophage elastase, oxidized alpha 1-PI was hardly degraded at all. Incubation of macrophage elastase with oxidized alpha 1-PI before the addition of native alpha 1-PI showed that oxidized alpha 1-PI was not an inhibitor of macrophage elastase. Competition experiments with up to twofold excess oxidized alpha 1-PI did not interfere with the degradation of native alpha 1-PI by macrophage elastase. Sequence analysis of amino acids in degraded native alpha 1-PI showed that macrophage elastase attacked a single peptide bond between Pro-357 and Met-358, the latter representing the P1 reactive-site residue of alpha 1-PI. In oxidized alpha 1-PI, Met-358 was converted to methionine sulfoxide and macrophage elastase hydrolyzed the bond between Phe-352 and Leu-353. These data suggest that methionine may be the primary cleavage site for macrophage elastase and not leucine, as previously thought.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-1082346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-110691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-1175627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-242232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-2989330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-2995374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-301739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-309470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-312289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-4104239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-4208353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-4429553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-447652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6059350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6153908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6193754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6427201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6428285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6432051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6604221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-6969773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-697847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-701239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-7030312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-7032505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-7038679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-938478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3494748-962868
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1314-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3494748-Amino Acid Sequence,
pubmed-meshheading:3494748-Animals,
pubmed-meshheading:3494748-Binding Sites,
pubmed-meshheading:3494748-Blood Proteins,
pubmed-meshheading:3494748-Humans,
pubmed-meshheading:3494748-Macrophages,
pubmed-meshheading:3494748-Mice,
pubmed-meshheading:3494748-Molecular Weight,
pubmed-meshheading:3494748-Oxidation-Reduction,
pubmed-meshheading:3494748-Pancreatic Elastase,
pubmed-meshheading:3494748-alpha 1-Antitrypsin
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pubmed:year |
1987
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pubmed:articleTitle |
Interaction of mouse macrophage elastase with native and oxidized human alpha 1-proteinase inhibitor.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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