Linked Life Data

3494736

Source:http://linkedlifedata.com/resource/pubmed/id/3494736

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-6-11
pubmed:abstractText
An interleukin-2 (IL-2) dependent murine cell line (TN-9) which could be grown continuously with the crude culture supernatant of concanavaline A-stimulated rat or mouse spleen cells could not synthesize DNA in the culture medium supplemented with partially purified or recombinant IL-2. The cell growth was restored by adding another factor obtained from the same crude culture supernatant. This factor, physicochemically inseparable from serum albumin, was also obtained from the culture medium added with 2-mercaptoethanol (2-ME) and incubated at 37 degrees C for 24 hr without the cells. By the experiments using semi-synchronized cell population, it was demonstrated that 2-ME or 2-ME carrying protein acted at the restricted process(es) of cell proliferation which occurred between IL-2-acting stage and the initiation of DNA synthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-8
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
2-Mercaptoethanol acts at the restricted stage of interleukin-2 dependent lymphocyte proliferation.
pubmed:publicationType
Journal Article