Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-4-6
pubmed:abstractText
The role of the T-cell-derived lymphokine B-cell stimulatory factor 1 (BSF-1) in the early activation, proliferation, and antibody-forming cell (AFC) clone formation of single fluorescein-specific B lymphocytes isolated from normal mouse spleens by hapten-gelatin adherence has been studied in vitro. BSF-1 acting alone induced early B-cell activation, as assessed by a significant increase in cell diameter of single B cells cultured for 24 hr. A small but significant number of these B cells formed proliferating clones, some of which secreted antibody. When acting with the specific antigen fluorescein-polymerized flagellin, BSF-1 augmented early cell enlargement and markedly enhanced proliferation, but it did not increase the frequency of AFC clones stimulated by fluorescein-polymerized flagellin alone. The further addition of recombinant murine interleukin 1 (IL-1) marginally enhanced proliferation caused by antigen plus BSF-1. No synergy was observed between BSF-1 and IL-1 for antibody formation. In the presence of fibroblast filler cells, BSF-1 substantially inhibited AFC clone development achieved by antigen plus IL-1. BSF-1 was also found to be inhibitory to AFC clone development stimulated by specific antigen acting with either recombinant human interleukin 2 (IL-2) or with IL-2 plus IL-1, both in the presence or absence of filler cells. The results suggest that BSF-1 plays a complex role in the regulation of the B-cell activation pathway by enhancing early activation and antigen-specific proliferation as well as inhibiting the effects of other B-cell factors on antibody formation. BSF-1 is the only cytokine so far tested in the single B-cell system that acts with antigen to promote proliferation without concomitant antibody production.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-2582266, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-2934482, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3005865, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3081637, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3083412, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3086481, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3090545, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3486902, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3487783, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-350592, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3873068, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3873071, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3877937, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3889907, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3905971, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3917279, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-3932582, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-57094, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6174979, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6190933, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6199407, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6209582, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6334854, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6352805, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6367046, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6434689, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6435125, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6439814, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6602200, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6977612, http://linkedlifedata.com/resource/pubmed/commentcorrection/3493492-6983069
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1389-93
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Single cell studies on the role of B-cell stimulatory factor 1 in B-cell activation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't