Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1987-4-21
|
| pubmed:abstractText |
Human interleukin 2 was detected at the pM level by a simple sequential sandwich enzyme immunoassay. The lymphokine to be assayed was first extracted from supernatants of mitogen-activated Jurkat leukemic T cells or peripheral blood lymphocytes using anti-recombinant interleukin 2 rabbit IgG insolubilized onto polystyrene microtiter plates and was revealed by an anti-interleukin 2 Fab' fragment conjugated to peroxidase. The whole method could be performed within 8 h and allowed the measurement of interleukin 2 irrespective of its degree of glycosylation. Among the currently used mitogens, only ConA at a concentration above 10 micrograms/ml interfered with the assay. The method was carefully compared to the reference bioassay and was found to be only 3-5 times less sensitive.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
12
|
| pubmed:volume |
97
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
215-20
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1987
|
| pubmed:articleTitle |
Human interleukin 2. Detection at the picomolar level by sandwich enzyme immunoassay.
|
| pubmed:publicationType |
Journal Article
|