Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1986-11-24
pubmed:abstractText
The transforming protein coded for by the Ki-ras oncogene, v-p21, localizes at the cytoplasmic face of the plasma membrane. A method is presented whereby the appearance of v-p21 in Kirsten murine sarcoma virus-transformed cells can be detected by flow cytometry, using a monoclonal antibody to v-p21 and methods modified from immunofluorescence microscopy. The method is sufficiently sensitive to differentiate between cellular and viral p21 levels, to detect small subpopulations of virus-transformed cells, and to monitor changes in p21 expression in response to physiologic variables. The method provides a rapid, quantitative means to investigate the expression of an intracellular transforming protein in heterogeneous cell populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0883-8364
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
621-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Detection of an intracellular transforming protein (v-Ki-ras p21) using the flow activated cell sorter (FACS).
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't