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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-9-18
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pubmed:abstractText |
P450c17 is the single enzyme mediating both 17 alpha-hydroxylase and 17,20 lyase activities. We identified several human P450c17 cDNA clones in a human adrenal cDNA library we constructed in lambda gt10. A short clone containing the 3'-terminal 650 bases of the full-length sequence was used to examine Southern blots of DNA from normal persons and from a panel of mouse/human somatic cell hybrid lines. The pattern of hybridization of this cDNA to normal human DNA cut with 8 restriction endonucleases suggests the human genome has two (or more) P450c17 genes. The pattern of hybridization to the somatic cell hybrid cell lines, each containing a limited, known number of human chromosomes, indicates the human adrenal P450c17 gene lies on chromosome 10. The chromosomal locations of the other P450c17 genes could not be determined.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-972X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-91
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3488328-Adrenal Glands,
pubmed-meshheading:3488328-Aldehyde-Lyases,
pubmed-meshheading:3488328-Animals,
pubmed-meshheading:3488328-Chromosome Mapping,
pubmed-meshheading:3488328-Chromosomes, Human, 6-12 and X,
pubmed-meshheading:3488328-Cytochrome P-450 Enzyme System,
pubmed-meshheading:3488328-DNA,
pubmed-meshheading:3488328-Humans,
pubmed-meshheading:3488328-Hybrid Cells,
pubmed-meshheading:3488328-Mice,
pubmed-meshheading:3488328-Nucleic Acid Hybridization,
pubmed-meshheading:3488328-Steroid 17-alpha-Hydroxylase,
pubmed-meshheading:3488328-Steroid Hydroxylases
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pubmed:year |
1986
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pubmed:articleTitle |
Assignment of the gene for adrenal P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase) to human chromosome 10.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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