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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1986-6-6
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pubmed:abstractText |
Proliferation of acute myeloblastic leukemia (AML) cells in vitro is limited in most cases to a small subset of blasts that have several properties of stem cells. These leukemic colony-forming cells (AML-CFU) generally require addition of exogenous growth factors for proliferation in agar or methylcellulose. These factors can be supplied by media conditioned by phytohemagglutinin-stimulated normal leukocytes or by CSF-secreting tumor cell lines. However, the exact factor or factors required for stimulation of AML-CFU growth have not been defined. We compared the AML-CFU stimulatory activity of a human recombinant GM-CSF with that of GCT-CM, Mo-CM, and the PHA-leukocyte feeder system in 15 cases of AML. In each of the 12 cases that required exogenous growth factors for maximum AML-CFU growth, recombinant GM-CSF could replace either GM-CSF or Mo-CM, and could partially replace the PHA-leukocyte feeder system. These results indicate that this GM-CSF is a growth promoter of AML-CFU in these culture systems.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
|
pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1448-53
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3486012-Adult,
pubmed-meshheading:3486012-Cell Division,
pubmed-meshheading:3486012-Cell Line,
pubmed-meshheading:3486012-Child,
pubmed-meshheading:3486012-Child, Preschool,
pubmed-meshheading:3486012-Clone Cells,
pubmed-meshheading:3486012-Colony-Stimulating Factors,
pubmed-meshheading:3486012-Culture Media,
pubmed-meshheading:3486012-Female,
pubmed-meshheading:3486012-Granulocytes,
pubmed-meshheading:3486012-Humans,
pubmed-meshheading:3486012-Leukemia, Myeloid, Acute,
pubmed-meshheading:3486012-Lymphocyte Activation,
pubmed-meshheading:3486012-Macrophages,
pubmed-meshheading:3486012-Male,
pubmed-meshheading:3486012-Middle Aged,
pubmed-meshheading:3486012-Phytohemagglutinins,
pubmed-meshheading:3486012-Recombinant Proteins
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pubmed:year |
1986
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pubmed:articleTitle |
Effects of recombinant human GM-CSF on proliferation of clonogenic cells in acute myeloblastic leukemia.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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