Linked Life Data

3485743

Source:http://linkedlifedata.com/resource/pubmed/id/3485743

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-5-19
pubmed:abstractText
The biochemical properties of an in-vitro megakaryocyte growth factor called megakaryocyte potentiator (Mk-POT) were investigated. P388D1 cell conditioned medium (P388D1 CM), was used as the source of Mk-POT. The potentiator activity had an apparent mol. wt of 21 kilodaltons (kd) by gel filtration and was eluted from DEAE-Sepharose pH 8.0 with 0.15 M NaCl. Chromatofocusing revealed three active species with apparent pIs of 4.0, 5.5 and above 6.0. Most Mk-POT activity does not bind to Concanavalin A-Sepharose. Mk-POT activity is sensitive to reduction by dithiothreitol and temperatures above 90 degrees C. Treatment with trypsin, alpha-chymotrypsin and pronase also reduced the Mk-POT activity, but it was not destroyed by RNase A or neuraminidase. It is precipitated in ammonium sulphate solutions of between 60 to 70% saturation, and by 80% ethanol. The Mk-POT activity is stable in solutions of pH 5.0-9.0. The data presented here suggest that megakaryocyte potentiator is either heterogeneous in its properties or more than one molecular species may express the in-vitro Mk-POT activity found in P388D1 CM.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0145-2126
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
403-11
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Biochemical characterization of an in-vitro murine megakaryocyte growth activity: megakaryocyte potentiator.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't