Linked Life Data

3485613

Source:http://linkedlifedata.com/resource/pubmed/id/3485613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-5-13
pubmed:abstractText
Primate (baboon) corneal endothelial cells have been grown continuously in vitro for over 40 passages. Endothelial cells migrated from the explants within three to five days. Growth became confluent by 14 to 21 days; the cells in culture were polygonal in shape and formed a compact monolayer. In passages one to four, cells divided with a doubling time of 72 hours; this increased to 96 hours after the 16th passage. Even though the general morphological appearance was unchanged in passages greater than 10, the cells became irregular, exhibiting an enlarged and elongated profile. The addition of epidermal growth factor to the medium resulted in increased cell growth. Cultured cells were successfully transplanted onto Descemet's membrane of de-endothelialized baboon corneas. This study demonstrates that primate corneal endothelial cells can be grown continuously in tissue culture and subsequently transplanted in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
607-11
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Growth characteristics of primate (baboon) corneal endothelium in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't