Linked Life Data

3480349

Source:http://linkedlifedata.com/resource/pubmed/id/3480349

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1988-2-2
pubmed:abstractText
The present study examined the orientation of cultured human gingival fibroblasts in simulated periodontal spaces in vitro containing three dimensional hydrated collagen gels. Extracted human teeth were root planed followed by root resection and root canal instrumentation. The middle and cervical thirds of each root were cut transversely to create 600-micron thick sections. Cortical bovine bone was cut, sectioned, and contoured to create bone rings 600 micron thick with an internal diameter large enough to accommodate a root slice leaving a circumferential space varying from approximately 0.1 to 1.0 mm. Root slices and bone rings were incubated in an enzyme solution to remove all remaining soft tissues and then completely demineralized in EDTA (18%) for 72 hours. Human gingival fibroblasts (HGF) were plated to confluency in tissue culture dishes. The dentin slices were then gently placed over the HGF monolayer along with bone rings around them to create simulated periodontal spaces. Five days later, when initial cell attachment to the dentin and root slices had occurred, a collagen gel was poured in the space. The cultures were maintained for six weeks and were then processed for transmission electron microscopy. The HGF appeared to have formed multilayered cell sheets extending from the periphery of the root slices to the inner surface of bone rings. The HGF had apparently attached to both the bone and root surfaces. There was a close interaction of cells with the matrix fibrils of the gel. The cells and matrix fibrils were oriented parallel to each other.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3492
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
762-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Orientation of gingival fibroblasts in simulated periodontal spaces in vitro containing collagen gels.
pubmed:affiliation
Department of Periodontics, School of Dentistry, University of North Carolina, Chapel Hill 27514.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.