Linked Life Data

3478200

Source:http://linkedlifedata.com/resource/pubmed/id/3478200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-12-14
pubmed:abstractText
The effect of various concentrations of 4 beta-phorbol dibutyrate, (4-beta PDBu) phorbol myristate acetate (PMA), and 4 beta-phorbol didecanoate (4 beta-PDD) were studied on the guinea-pig parenchymal strip. The order of potency, 4 beta-PDBu greater than PMA greater than 4 beta-PDD, was the inverse of their lipid solubility. 4 beta-PDBu, 10(-9)-10(-4) M, caused a powerful, slow, sustained contraction starting within 2-3 min and reaching maximum in approximately 45 min, the maximum being 170% of the maximum histamine contraction. The responses to PMA and 4 beta-PDD were slower and less marked. 4 alpha-Phorbol dibutyrate had no effect. These results differ from those reported for guinea-pig trachea. In calcium-free Krebs solution + EGTA (1 mM) the cumulative concentration-response curve to 4 beta-PDBu was still obtained but was slower and was diminished. A23187, ionomycin and vanadate also caused contraction, the respective concentration ranges being 10(-7)-10(-5), 10(-8)-10(-6) and 10(-6)-10(-3) M. When a low concentration of 4 beta-PDBu, which on its own produced no effect, was given with a low concentration of A23187, ionomycin or vanadate, marked synergism was seen. These results are consistent with the model of smooth muscle contraction in which it was proposed that the initial response to stimulation is mediated by both the trisphosphate/calcium and diacylglycerol/protein kinase C pathways, while the sustained response is mediated by the diacylglycerol/protein kinase C pathway, only.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
23-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
4 beta-PDBu contracts parenchymal strip and synergizes with raised cytosolic calcium.
pubmed:affiliation
Department of Pharmacology, University College London, U.K.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't