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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1987-10-15
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pubmed:abstractText |
Human chorionic gonadotropin (hCG) is a member of a family of heterodimeric glycoprotein hormones that have a common alpha subunit but differ in their hormone-specific beta subunits. The beta subunit of hCG (hCG beta) is unique among the beta subunits in that it contains four mucin-like O-linked oligosaccharides attached to a carboxyl-terminal extension. To study the effects of O-glycosylation on the secretion and assembly of hCG, expression vectors containing either the hCG beta gene alone or together with the hCG alpha gene were transfected into a mutant Chinese hamster ovary cell line, IdID, which exhibits a reversible defect in O-glycosylation. Our results reveal that hCG beta can be secreted normally in the absence of its O-linked oligosaccharides. hCG beta devoid of O-linked carbohydrate can also combine efficiently with hCG alpha and be secreted as an intact dimer. We conclude that in Chinese hamster ovary cells, the hCG beta O-linked chains play no role in the assembly and secretion of hCG. The normal and O-linked oligosaccharide-deficient forms of hCG secreted by these cells should prove useful in examining the role of O-linked chains on the biological function of hCG.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-225559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-2437127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-3104337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-3948246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-4685398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-468798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-4795659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6194155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6210286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6267583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6267989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6299584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6317691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6321999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6327272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6774259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6812050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-6961440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-838693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-885857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3476951-885858
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6354-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3476951-Acetylgalactosamine,
pubmed-meshheading:3476951-Animals,
pubmed-meshheading:3476951-Cell Line,
pubmed-meshheading:3476951-Chorionic Gonadotropin,
pubmed-meshheading:3476951-Cloning, Molecular,
pubmed-meshheading:3476951-Cricetinae,
pubmed-meshheading:3476951-Glycoproteins,
pubmed-meshheading:3476951-Glycosylation,
pubmed-meshheading:3476951-Humans,
pubmed-meshheading:3476951-Protein Processing, Post-Translational
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pubmed:year |
1987
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pubmed:articleTitle |
Effects of preventing O-glycosylation on the secretion of human chorionic gonadotropin in Chinese hamster ovary cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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