|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1987-8-31
|
|
pubmed:abstractText |
Platelet-derived growth factor (PDGF) is one of the most important polypeptide growth factors in human serum. It is composed of two polypeptide chains linked by disulfide bonds. The B-chain is encoded by the c-sis proto-oncogene, which is expressed in several malignant and non-malignant cells including K562 cells differentiating towards megakaryoblasts. Expression of the A-chain has been reported to occur in human solid tumor cell lines independently of c-sis expression. We report here the non-coordinate expression of the A- and B-chains in human leukemia cell lines. The PDGF-A and B-chain (c-sis) RNA expression as well as secretion of PDGF polypeptides are induced in the K562 cell line upon induction of megakaryoblastic differentiation with 12-O-tetradecanoyl phorbol-13-acetate (TPA) whereas erythroid differentiation induced with sodium butyrate is accompanied by c-sis expression only. Simultaneously with megakaryoblastic differentiation the RNA level for another platelet protein, the transforming growth factor-beta was also increased, but in a complex manner. The promyelocytic leukemia cell line HL-60 does not express PDGF-A RNA, whereas the promonocytic cell line U937 does. Preferential induction of the A-chain RNA is obtained in both cell lines after treatment with TPA which causes monocytic differentiation. PDGF-A expression in HL-60 cells is also observed after treatment with the tumor necrosis factor-alpha but granulocytic differentiation of HL-60 cells induced with dimethyl sulfoxide or the granulocyte colony-stimulating factor is not associated with PDGF gene expression.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-163658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-178611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2408759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2409450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2416458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2425362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-276884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-288066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2941150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2983887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2984061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2986848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2989692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-2990725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3007454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3011421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3013421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3023859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3085953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3095282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3456591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3456608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3463866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3466024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3520340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3529084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3538015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3754619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-379005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3861940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3887183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-3941744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-4033772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-570644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6171440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6198073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6204766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6208557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6227332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6232432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6232493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6257398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6265924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6304883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6306471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6308652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6349795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6373015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6387712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6479151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6499844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6602130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6689055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6943569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-6954533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3475202-95354
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
6
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1213-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3475202-Humans,
pubmed-meshheading:3475202-Leukemia, Myeloid,
pubmed-meshheading:3475202-Monocytes,
pubmed-meshheading:3475202-Genes,
pubmed-meshheading:3475202-Megakaryocytes,
pubmed-meshheading:3475202-Cell Differentiation,
pubmed-meshheading:3475202-RNA, Messenger,
pubmed-meshheading:3475202-Cell Line,
pubmed-meshheading:3475202-Gene Expression Regulation
|
